rabbit-polyclonal anti nr2f1  (Proteintech)

Journal: Journal of Cerebral Blood Flow & Metabolism

Article Title: Sonic hedgehog is expressed in human brain arteriovenous malformations and induces arteriovenous malformations in vivo

doi: 10.1177/0271678x20912405

Figure Lengend Snippet: Figure 1. Immunohistochemical analysis of Shh pathway expression in human brain AVMs. Representative images of human brain AVMs and control brain vessels. Cells in the intimal layer of AVMs are strongly immunopositive for Shh and Gli1. COUP-TFII immunopositive cells are detectable in the intimal and medial layers of AVMs. Control brain vessels are negative in terms of Shh, Gli1, and COUP-TFII expression. Shh: Sonic hedgehog.

Article Snippet: For the detection of COUP-TFII expression in brain vasculature, sections were incubated with lectin from Lycopersicon esculentum (tomato) biotin conjugate (1:500) (SigmaAldrich, St. Louis, MO, USA) together with a rabbit polyclonal anti-COUP-TFII antibody (Origene, Rockville, MD, USA).

Techniques: Immunohistochemical staining, Expressing, Control

Journal: Journal of Cerebral Blood Flow & Metabolism

Article Title: Sonic hedgehog is expressed in human brain arteriovenous malformations and induces arteriovenous malformations in vivo

doi: 10.1177/0271678x20912405

Figure Lengend Snippet: Figure 7. Expression of COUP-TFII in phShh-induced brain neovessels. Sections of brain hemispheres injected with phShh and empty plasmid were stained for DAPI (blue staining) to identify cell nuclei, lectin (red staining) to identify blood vessels, and COUP- TFII (green staining) to identify cells expressing this vascular differentiation marker. (a) COUP-TFII-positive cells were found in the neovessels grown in the brain in response to phShh injection, both at the level of the intimal layer and the vascular wall. (b) COUP- TFII-positive cells were virtually absent in brain hemispheres injected with the empty plasmid.

Article Snippet: For the detection of COUP-TFII expression in brain vasculature, sections were incubated with lectin from Lycopersicon esculentum (tomato) biotin conjugate (1:500) (SigmaAldrich, St. Louis, MO, USA) together with a rabbit polyclonal anti-COUP-TFII antibody (Origene, Rockville, MD, USA).

Techniques: Expressing, Injection, Plasmid Preparation, Staining, Marker